| First Author | Sabik OL | Year | 2017 |
| Journal | G3 (Bethesda) | Volume | 7 |
| Issue | 3 | Pages | 865-870 |
| PubMed ID | 28082324 | Mgi Jnum | J:244516 |
| Mgi Id | MGI:5913295 | Doi | 10.1534/g3.116.037424 |
| Citation | Sabik OL, et al. (2017) Genetic Dissection of a QTL Affecting Bone Geometry. G3 (Bethesda) 7(3):865-870 |
| abstractText | Parameters of bone geometry such as width, length, and cross-sectional area are major determinants of bone strength. Although these traits are highly heritable, few genes influencing bone geometry have been identified. Here, we dissect a major quantitative trait locus (QTL) influencing femur size. This QTL was originally identified in an F2 cross between the C57BL/6J-hg/hg (HG) and CAST/EiJ strains and was referred to as femur length in high growth mice 2 (Feml2). Feml2 was located on chromosome (Chr.) 9 at approximately 20 cM. Here, we show that the HG.CAST-(D9Mit249-D9Mit133)/Ucd congenic strain captures Feml2 In an F2 congenic cross, we fine-mapped the location of Feml2 to an approximately 6 Mbp region extending from 57.3 to 63.3 Mbp on Chr. 9. We have identified candidates by mining the complete genome sequence of CAST/EiJ and through allele-specific expression (ASE) analysis of growth plates in C57BL/6J x CAST/EiJ F1 hybrids. Interestingly, we also find that the refined location of Feml2 overlaps a cluster of six independent genome-wide associations for human height. This work provides the foundation for the identification of novel genes affecting bone geometry. |
