| Primary Identifier | MGI:2386269 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ikbkb |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Two exons encoding amino acids 36-106 of the kinase domain were replaced with a PGK-neo cassette via homologous recombination. The targeted mutation destroys lyine 44, a residue necessary for kinase activity. Homozygous mutant embryos were detected by PCR genotype analysis up to day E14.5. Sequence analysis of RT-PCR products from mouse embryonic fibroblasts (derived from E12.2 homozygous mutant embryos) revealed an in-frame deletion from nucleotides 106-318. However, Western blot analysis using antibodies directed against the C-terminus of Ikbkb failed to detect protein product. |
