| First Author | Kittaka M | Year | 2020 |
| Journal | JBMR Plus | Volume | 4 |
| Issue | 6 | Pages | e10352 |
| PubMed ID | 32537546 | Mgi Jnum | J:360238 |
| Mgi Id | MGI:7797566 | Doi | 10.1002/jbm4.10352 |
| Citation | Kittaka M, et al. (2020) Microbe-Dependent Exacerbated Alveolar Bone Destruction in Heterozygous Cherubism Mice. JBMR Plus 4(6):e10352 |
| abstractText | Cherubism (OMIM#118400) is a craniofacial disorder characterized by destructive jaw expansion. Gain-of-function mutations in SH3-domain binding protein 2 (SH3BP2) are responsible for this rare disorder. We have previously shown that homozygous knock-in (KI) mice (Sh3bp2 (KI/KI) ) recapitulate human cherubism by developing inflammatory lesions in the jaw. However, it remains unknown why heterozygous KI mice (Sh3bp2 (KI/+) ) do not recapitulate the excessive jawbone destruction in human cherubism, even though all mutations are heterozygous in humans. We hypothesized that Sh3bp2 (KI/+) mice need to be challenged for developing exacerbated jawbone destruction and that bacterial stimulation in the oral cavity may be involved in the mechanism. In this study, we applied a ligature-induced periodontitis model to Sh3bp2 (KI/+) mice to induce inflammatory alveolar bone destruction. Ligature placement induced alveolar bone resorption with gingival inflammation. Quantification of alveolar bone volume revealed that Sh3bp2 (KI/+) mice developed more severe bone loss (male: 43.0% +/- 10.6%, female: 42.6% +/- 10.4%) compared with Sh3bp2 (+/+) mice (male: 25.8% +/- 4.0%, female: 30.9% +/- 6.5%). Measurement of bone loss by the cement-enamel junction-alveolar bone crest distance showed no difference between Sh3bp2 (KI/+) and Sh3bp2 (+/+) mice. The number of osteoclasts on the alveolar bone surface was higher in male Sh3bp2 (KI/+) mice, but not in females, compared with Sh3bp2 (+/+) mice. In contrast, inflammatory cytokine levels in gingiva were comparable between Sh3bp2 (KI/+) and Sh3bp2 (+/+) mice with ligatures. Genetic deletion of the spleen tyrosine kinase in myeloid cells and antibiotic treatment suppressed alveolar bone loss in Sh3bp2 (KI/+) mice, suggesting that increased osteoclast differentiation and function mediated by SYK and accumulation of oral bacteria are responsible for the increased alveolar bone loss in Sh3bp2 (KI/+) mice with ligature-induced periodontitis. High amounts of oral bacterial load caused by insufficient oral hygiene could be a trigger for the initiation of jawbone destruction in human cherubism. (c) 2020 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research. |
