| First Author | Boulais PE | Year | 2018 |
| Journal | Immunity | Volume | 49 |
| Issue | 4 | Pages | 627-639.e6 |
| PubMed ID | 30314756 | Mgi Jnum | J:273617 |
| Mgi Id | MGI:6284429 | Doi | 10.1016/j.immuni.2018.08.019 |
| Citation | Boulais PE, et al. (2018) The Majority of CD45(-) Ter119(-) CD31(-) Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors. Immunity 49(4):627-639.e6 |
| abstractText | The non-hematopoietic cell fraction of the bone marrow (BM) is classically identified as CD45(-) Ter119(-) CD31(-) (herein referred to as triple-negative cells or TNCs). Although TNCs are believed to contain heterogeneous stromal cell populations, they remain poorly defined. Here we showed that the vast majority of TNCs ( approximately 85%) have a hematopoietic rather than mesenchymal origin. Single cell RNA-sequencing revealed erythroid and lymphoid progenitor signatures among CD51(-) TNCs. Ly6D(+) CD44(+) CD51(-) TNCs phenotypically and functionally resembled CD45(+) pro-B lymphoid cells, whereas Ly6D(-) CD44(+) CD51(-) TNCs were enriched in previously unappreciated stromal-dependent erythroid progenitors hierarchically situated between preCFU-E and proerythroblasts. Upon adoptive transfer, CD44(+) CD51(-) TNCs contributed to repopulate the B-lymphoid and erythroid compartments. CD44(+) CD51(-) TNCs also expanded during phenylhydrazine-induced acute hemolysis or in a model of sickle cell anemia. These findings thus uncover physiologically relevant new classes of stromal-associated functional CD45(-) hematopoietic progenitors. |
