| First Author | Mohrmann L | Year | 2024 |
| Journal | Int J Mol Sci | Volume | 25 |
| Issue | 2 | PubMed ID | 38279285 |
| Mgi Jnum | J:358307 | Mgi Id | MGI:7579928 |
| Doi | 10.3390/ijms25021285 | Citation | Mohrmann L, et al. (2024) Distinct Alterations in Dendritic Spine Morphology in the Absence of beta-Neurexins. Int J Mol Sci 25(2) |
| abstractText | Dendritic spines are essential for synaptic function because they constitute the postsynaptic compartment of the neurons that receives the most excitatory input. The extracellularly shorter variant of the presynaptic cell adhesion molecules neurexins, beta-neurexin, has been implicated in various aspects of synaptic function, including neurotransmitter release. However, its role in developing or stabilizing dendritic spines as fundamental computational units of excitatory synapses has remained unclear. Here, we show through morphological analysis that the deletion of beta-neurexins in hippocampal neurons in vitro and in hippocampal tissue in vivo affects presynaptic dense-core vesicles, as hypothesized earlier, and, unexpectedly, alters the postsynaptic spine structure. Specifically, we observed that the absence of beta-neurexins led to an increase in filopodial-like protrusions in vitro and more mature mushroom-type spines in the CA1 region of adult knockout mice. In addition, the deletion of beta-neurexins caused alterations in the spine head dimension and an increase in spines with perforations of their postsynaptic density but no changes in the overall number of spines or synapses. Our results indicate that presynaptic beta-neurexins play a role across the synaptic cleft, possibly by aligning with postsynaptic binding partners and glutamate receptors via transsynaptic columns. |
