| First Author | Hatton RD | Year | 2006 |
| Journal | Immunity | Volume | 25 |
| Issue | 5 | Pages | 717-29 |
| PubMed ID | 17070076 | Mgi Jnum | J:116207 |
| Mgi Id | MGI:3693168 | Doi | 10.1016/j.immuni.2006.09.007 |
| Citation | Hatton RD, et al. (2006) A distal conserved sequence element controls Ifng gene expression by T cells and NK cells. Immunity 25(5):717-29 |
| abstractText | Chromatin dynamics that regulate Ifng gene expression are incompletely understood. By using cross-species comparative sequence analyses, we have identified conserved noncoding sequences (CNSs) upstream of the Ifng gene, one of which, located -22 kb from the transcriptional start site, contains clustered consensus binding sequences of transcription factors that function in T cell differentiation. CNS-22 was uniquely associated with histone modifications typical of accessible chromatin in both T helper 1 (Th1) and Th2 cells and demonstrated significant and selective T-bet (T-box transcription factor expressed in T cells, Tbx21)-dependent binding and enhancer activity in Th1 cells. Deletion of CNS-22 in the context of an Ifng reporter transgene ablated T cell receptor-dependent and -independent Ifng expression in Th1 effectors and similarly blocked expression by cytotoxic T lymphocytes and natural killer cells. Thus, a single distal element may be essential for Ifng gene expression by both innate and adaptive immune effector cell lineages. |
