| First Author | Wu X | Year | 2007 |
| Journal | Genesis | Volume | 45 |
| Issue | 10 | Pages | 653-7 |
| PubMed ID | 17941048 | Mgi Jnum | J:128709 |
| Mgi Id | MGI:3767895 | Doi | 10.1002/dvg.20339 |
| Citation | Wu X, et al. (2007) Generation of conditional knockout alleles for PDGF-C. Genesis 45(10):653-7 |
| abstractText | PDGF-C is a newly identified member of the platelet-derived growth factor (PDGF) family, which is involved in multiple cellular functions by signaling through PDGF receptor (PDGFR)-alphaalpha and alphabeta dimers. PDGF-C deficiency is perinatal lethal due to the formation of cleft palate. To further characterize the cellular function of PDGF-C during both embryonic and postnatal development, we have generated two conditional alleles of the Pdgf-c gene in which two loxP sites flank exon 5. Global Cre-mediated excision of the floxed exon 5 in these alleles resulted in a complete loss of PDGF-C expression and caused embryonic defects identical to those previously described for the PDGF-C null embryos. These conditional alleles will therefore be the important genetic tools for dissecting the spatial and temporal roles of PDGF-C during development and in adult tissues. Furthermore, from this work, we have also described a simple approach for creating mouse conditional alleles in an efficient manner. |
