| First Author | Roux I | Year | 2016 |
| Journal | J Neurophysiol | Volume | 116 |
| Issue | 2 | Pages | 479-92 |
| PubMed ID | 27098031 | Mgi Jnum | J:244196 |
| Mgi Id | MGI:5912975 | Doi | 10.1152/jn.01038.2015 |
| Citation | Roux I, et al. (2016) Assessment of the expression and role of the alpha1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea. J Neurophysiol 116(2):479-92 |
| abstractText | Hair cell (HC) activity in the mammalian cochlea is modulated by cholinergic efferent inputs from the brainstem. These inhibitory inputs are mediated by calcium-permeable nicotinic acetylcholine receptors (nAChRs) containing alpha9- and alpha10-subunits and by subsequent activation of calcium-dependent potassium channels. Intriguingly, mRNAs of alpha1- and gamma-nAChRs, subunits of the "muscle-type" nAChR have also been found in developing HCs (Cai T, Jen HI, Kang H, Klisch TJ, Zoghbi HY, Groves AK. J Neurosci 35: 5870-5883, 2015; Scheffer D, Sage C, Plazas PV, Huang M, Wedemeyer C, Zhang DS, Chen ZY, Elgoyhen AB, Corey DP, Pingault V. J Neurochem 103: 2651-2664, 2007; Sinkkonen ST, Chai R, Jan TA, Hartman BH, Laske RD, Gahlen F, Sinkkonen W, Cheng AG, Oshima K, Heller S. Sci Rep 1: 26, 2011) prompting proposals that another type of nAChR is present and may be critical during early synaptic development. Mouse genetics, histochemistry, pharmacology, and whole cell recording approaches were combined to test the role of alpha1-nAChR subunit in HC efferent synapse formation and cholinergic function. The onset of alpha1-mRNA expression in mouse HCs was found to coincide with the onset of the ACh response and efferent synaptic function. However, in mouse inner hair cells (IHCs) no response to the muscle-type nAChR agonists (+/-)-anatoxin A, (+/-)-epibatidine, (-)-nicotine, or 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) was detected, arguing against the presence of an independent functional alpha1-containing muscle-type nAChR in IHCs. In alpha1-deficient mice, no obvious change of IHC efferent innervation was detected at embryonic day 18, contrary to the hyperinnervation observed at the neuromuscular junction. Additionally, ACh response and efferent synaptic activity were detectable in alpha1-deficient IHCs, suggesting that alpha1 is not necessary for assembly and membrane targeting of nAChRs or for efferent synapse formation in IHCs. |
