| First Author | Joo JH | Year | 2007 |
| Journal | Dev Dyn | Volume | 236 |
| Issue | 8 | Pages | 2147-58 |
| PubMed ID | 17654715 | Mgi Jnum | J:123288 |
| Mgi Id | MGI:3717952 | Doi | 10.1002/dvdy.21243 |
| Citation | Joo JH, et al. (2007) Role of Pinin in neural crest, dorsal dermis, and axial skeleton development and its involvement in the regulation of Tcf/Lef activity in mice. Dev Dyn 236(8):2147-58 |
| abstractText | Previous in vitro studies have indicated multiple and varied roles of Pinin (PNN); however, its in vivo role has remained unclear. Here, we report generation of null, hypomorphic, and conditional Pnn alleles in mice. We found that insertion of neomycin-resistance cassette into intron 8 of Pnn resulted in knockdown of Pnn, which allowed Pnn hypomorphic embryos to pass peri-implantation lethality. These mice are lethal at perinatal stages and exhibit defects in the cardiac outflow tract, palate, dorsal dermis, and axial skeleton. Since Wnt/beta-catenin signaling has been shown to play pivotal roles in development of all tissues affected by Pnn hypomorphism, we speculated that Pnn may affect Wnt/beta-catenin signaling. Supporting this view, we demonstrate abnormal activities of Tcf/Lef transcription factors, and alterations in beta-catenin level in multiple Pnn hypomorphic tissues. Taken together, the data suggest that Pnn plays important roles during mouse development through its involvement in regulation of Tcf/Lef activity. Developmental Dynamics 236:2147-2158, 2007. (c) 2007 Wiley-Liss, Inc. |
