| First Author | Sene A | Year | 2009 |
| Journal | PLoS One | Volume | 4 |
| Issue | 10 | Pages | e7329 |
| PubMed ID | 19809515 | Mgi Jnum | J:154103 |
| Mgi Id | MGI:4367287 | Doi | 10.1371/journal.pone.0007329 |
| Citation | Sene A, et al. (2009) Functional implication of Dp71 in osmoregulation and vascular permeability of the retina. PLoS One 4(10):e7329 |
| abstractText | Functional alterations of Muller cells, the principal glia of the retina, are an early hallmark of most retina diseases and contribute to their further progression. The molecular mechanisms of these reactive Muller cell alterations, resulting in disturbed retinal homeostasis, remain largely unknown. Here we show that experimental detachment of mouse retina induces mislocation of the inwardly rectifying potassium channels (Kir4.1) and a downregulation of the water channel protein (AQP4) in Muller cells. These alterations are associated with a strong decrease of Dp71, a cytoskeleton protein responsible for the localization and the clustering of Kir4.1 and AQP4. Partial (in detached retinas) or total depletion of Dp71 in Muller cells (in Dp71-null mice) impairs the capability of volume regulation of Muller cells under osmotic stress. The abnormal swelling of Muller cells In Dp71-null mice involves the action of inflammatory mediators. Moreover, we investigated whether the alterations in Muller cells of Dp71-null mice may interfere with their regulatory effect on the blood-retina barrier. In the absence of Dp71, the retinal vascular permeability was increased as compared to the controls. Our results reveal that Dp71 is crucially implicated in the maintenance of potassium homeostasis, in transmembraneous water transport, and in the Muller cell-mediated regulation of retinal vascular permeability. Furthermore, our data provide novel insights into the mechanisms of retinal homeostasis provided by Muller cells under normal and pathological conditions. |
