| First Author | Cody DD | Year | 2005 |
| Journal | Invest Radiol | Volume | 40 |
| Issue | 5 | Pages | 263-9 |
| PubMed ID | 15829823 | Mgi Jnum | J:98655 |
| Mgi Id | MGI:3579595 | Doi | 10.1097/01.rli.0000160070.67270.05 |
| Citation | Cody DD, et al. (2005) Murine Lung Tumor Measurement Using Respiratory-Gated Micro-Computed Tomography. Invest Radiol 40(5):263-269 |
| abstractText | OBJECTIVE:: The authors explored micro-computed tomography (micro-CT) to quantify lung tumor number and volume in a specific genetic mouse model for lung cancer. MATERIALS AND METHODS:: The authors used K-ras mice, which develop lung adenomas and adenocarcinomas through somatic activation of the K-ras oncogene. Tumor number measured using micro-CT and were compared at necropsy (n = 38 mice). Tumor volume measurement precision (n = 39 mice) and accuracy (multiple tumors from a single mouse) were evaluated. Serial lung tumor volume was assessed in a pilot group (n = 8) of mice in vivo. RESULTS:: Tumor number assessed at necropsy and using micro-CT were significantly correlated. Lung tumor volume measurements were both reproducible (2% operator variability) and accurate (6% average error). Strikingly, we observed both tumor growth and shrinkage within individual mice. CONCLUSION:: Serial measurements provided evidence of tumor heterogeneity, an unexpected finding given the uniformity of the initiating genetic event. Micro-CT may become a powerful tool for murine lung cancer research in vivo. |
