| First Author | Farache J | Year | 2013 |
| Journal | Immunity | Volume | 38 |
| Issue | 3 | Pages | 581-95 |
| PubMed ID | 23395676 | Mgi Jnum | J:194474 |
| Mgi Id | MGI:5473918 | Doi | 10.1016/j.immuni.2013.01.009 |
| Citation | Farache J, et al. (2013) Luminal Bacteria Recruit CD103(+) Dendritic Cells into the Intestinal Epithelium to Sample Bacterial Antigens for Presentation. Immunity 38(3):581-95 |
| abstractText | CD103(+) dendritic cells (DCs) carry bacteria from the small intestine and can present antigens to T cells. Yet they have not been recorded sampling luminal bacteria or presenting bacterial antigens in mesentery lymph nodes. We used 2-photon microscopy in live Cx3cr1(+/gfp) xCd11c-YFP mice to study these processes. At steady state, sparse CD103(+) DCs occupied the epithelium. They patrolled among enterocytes while extending dendrites toward the lumen, likely using tight-junction proteins to penetrate the epithelium. Challenge with Salmonella triggered chemokine- and toll-like receptor (TLR)-dependent recruitment of additional DCs from the lamina propria (LP). The DCs efficiently phagocytosed the bacteria using intraepithelial dendrites. Noninvasive bacteria were similarly sampled. In contrast, CD103(+) DCs sampled soluble luminal antigen inefficiently. In mice harboring CD103(+) DCs, antigen-specific CD8 T cells were subsequently activated in MLNs. Intestinal CD103(+) DCs are therefore equipped with unique mechanisms to independently complete the processes of uptake, transportation, and presentation of bacterial antigens. |
