| First Author | Chin SS | Year | 2022 |
| Journal | Nat Commun | Volume | 13 |
| Issue | 1 | Pages | 2240 |
| PubMed ID | 35474218 | Mgi Jnum | J:326923 |
| Mgi Id | MGI:7265918 | Doi | 10.1038/s41467-022-29718-2 |
| Citation | Chin SS, et al. (2022) T cell receptor and IL-2 signaling strength control memory CD8(+) T cell functional fitness via chromatin remodeling. Nat Commun 13(1):2240 |
| abstractText | Cognate antigen signal controls CD8(+) T cell priming, expansion size and effector versus memory cell fates, but it is not known if and how it modulates the functional features of memory CD8(+) T cells. Here we show that the strength of T cell receptor (TCR) signaling controls the requirement for interleukin-2 (IL-2) signals to form a pool of memory CD8(+) T cells that competitively re-expand upon secondary antigen encounter. Combining strong TCR and intact IL-2 signaling during priming synergistically induces genome-wide chromatin accessibility in regions targeting a wide breadth of biological processes, consistent with greater T cell functional fitness. Chromatin accessibility in promoters of genes encoding for stem cell, cell cycle and calcium-related proteins correlates with faster intracellular calcium accumulation, initiation of cell cycle and more robust expansion. High-dimensional flow-cytometry analysis of these T cells also highlights higher diversity of T cell subsets and phenotypes with T cells primed with stronger TCR and IL-2 stimulation than those primed with weaker strengths of TCR and/or IL-2 signals. These results formally show that epitope selection in vaccine design impacts memory CD8(+) T cell epigenetic programming and function. |
