| First Author | Dhanya SK | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 1 | Pages | 101105 |
| PubMed ID | 35098161 | Mgi Jnum | J:350225 |
| Mgi Id | MGI:7661239 | Doi | 10.1016/j.xpro.2021.101105 |
| Citation | Dhanya SK, et al. (2022) Two photon imaging of calcium responses in murine Purkinje neurons. STAR Protoc 3(1):101105 |
| abstractText | Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca(2+) transients can be challenging. Here, we describe a protocol for measuring Ca(2+) transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca(2+) signaling in cerebellar slices expressing a genetic Ca(2+) reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021). |
