| Primary Identifier | MGI:2137832 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ptgir |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Loss of Ptgir was shown functionally: e.g., a prostacyclin analogue (cicaprost) was used to inhibit platelet aggregation induced by ADP (platelet aggregation was inhibited in wild-type mice, but not in homozygous mutant mice), relaxation of smooth muscle in aortic rings of wild-type and homozygous mutant mice was measured after stimulation with a prostacyclin analogue (cicaprost, again, homozygous mutant mice showed no relaxation upon stimulation). Specificity of the knock-out was demonstrated by functional assay using other prostaglandins (e.g., PGE2). No differences between wild-type and homozygous mutant deficient mice were seen in response to PGE2. |
| molecularNote | A neomycin resistance cassette replaced 2.4kb of sequence containing parts of the putative exons II and III, which encode the sixth transmembrane domain. A functional assay did not detect the protein in homozygous mutant mice. |
