| Primary Identifier | MGI:5548082 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | The V6.5 embryonic stem (ES) cells used in the making of the mouse were targeted with a GT(ROSA)26Sor targeting vector containing an F3- and FRT-flanked ATG-F3-Puro-pA cassette followed by a PGK promoter driving expression of FLPo recombinase. |
| molecularNote | A recombinase mediated exchange (RMCE) vector was designed to contain (from 5' to 3') a promoterless neomycin cassette followed by 3 SV40 polyadenylation sites (pA), 3xpA, and in reverse orientation a fragment containing 3xIns (400 bp core region of chicken beta-globin insulator), CAGGS (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin) hybrid promoter, floxed-3xpA, and a cDNA encoding an enhanced green fluorescent protein (EGFP) fused to ribosomal protein unit L10a (Rpl10a). The loxP-flanked pA cassette is designed to block transcription of EGFP/Rpl10a. |
