| First Author | Al Sweidi S | Year | 2013 |
| Journal | Neuroscience | Volume | 236 |
| Pages | 99-109 | PubMed ID | 23357113 |
| Mgi Jnum | J:201379 | Mgi Id | MGI:5513079 |
| Doi | 10.1016/j.neuroscience.2012.12.058 | Citation | Al Sweidi S, et al. (2013) Estrogen receptors and lesion-induced response of striatal dopamine receptors. Neuroscience 236:99-109 |
| abstractText | Neuroprotection by 17beta-estradiol and an estrogen receptor (ER) agonist against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) lesion were shown to implicate protein kinase B (Akt) signaling in mice. In order to evaluate the associated mechanisms, this study compared estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) intact or knockout (KO) and wild-type (WT) C57Bl/6 male mice following MPTP treatment of 7, 9, 11mg/kg and/or 17beta-estradiol. Striatal D1 and D2 dopamine (DA) receptors were measured by autoradiography with the specific ligands [(3)H]-SCH 23390 and [(3)H]-raclopride, respectively and signaling by Western blot for Akt, glycogen synthase kinase 3beta (GSK3beta) and extracellular-regulated signal kinases (ERK1 and ERK2). Control ERKObeta mice had lower striatal [(3)H]-SCH 23390 specific binding than WT and ERKOalpha mice; both KO mice had lower [(3)H]-raclopride specific binding. Striatal D1 receptors decreased with increasing doses of MPTP in correlation with striatal DA concentrations in ERKOalpha mice and remained unchanged in WT and ERKObeta mice. Striatal D2 receptors decreased with increasing doses of MPTP in correlation with striatal DA concentrations in WT and ERKOalpha mice and increased in ERKObeta mice. In MPTP-lesioned mice, 17beta-estradiol treatment increased D1 receptors in ERKOalpha and ERKObeta mice and D2 receptors in WT and ERKObeta mice. MPTP did not affect striatal pAkt/Akt and pGSK3beta/GSK3beta levels in WT and ERKOalpha mice, while in vehicle-treated ERKObeta mice these levels were higher and increased with MPTP lesioning. Striatal pERK1/ERK1 and pERK2/ERK2 levels showed to a lesser extent a similar pattern. In conclusion, ERs affected the response of striatal DA receptors to a MPTP lesion and post receptor signaling. |
