| First Author | Dominguez R | Year | 2010 |
| Journal | J Neurosci | Volume | 30 |
| Issue | 38 | Pages | 12589-96 |
| PubMed ID | 20861365 | Mgi Jnum | J:164653 |
| Mgi Id | MGI:4834932 | Doi | 10.1523/JNEUROSCI.1038-10.2010 |
| Citation | Dominguez R, et al. (2010) Estradiol rapidly regulates membrane estrogen receptor alpha levels in hypothalamic neurons. J Neurosci 30(38):12589-96 |
| abstractText | Estrogen receptors (ERs) and estrogen-binding proteins have been localized intracellularly and on the cell surface. The membrane-associated proteins initiate signaling that activates a myriad of cellular responses including the modulation of ion channels and ultimately transcription. Although many of the downstream actions of membrane ERs, including ERalpha and ERbeta, have been characterized, the mechanisms regulating membrane ER levels have remained elusive in the nervous system. In the present study, we used surface biotinylation to identify and study the estradiol regulation of membrane ERalpha in mixed-sex, cultured hypothalamic neurons from rat. Following surface biotinylation, Western blot analysis revealed full-length 66 kDa ERalpha and several ERalpha splice variants, most notably a biotinylated 52 kDa ERalpha-immunoreactive protein. Treatment of the neurons with estradiol caused a rapid and transient increase of the biotinylated 52 kDa and 66 kDa ERalpha proteins in the plasma membrane. Exposure of the neurons to estradiol also significantly increased internalization of 52 kDa and 66 kDa ERalpha membrane proteins, a measure of receptor activation. In the hypothalamus, membrane ERalpha signaling depends on transactivation of metabotropic glutamate receptor-1a (mGluR1a). Estradiol treatment increased the internalization of mGluR1a in parallel with ERalpha, a finding consistent with the hypothesis of an ERalpha-mGluR1a signaling unit. These results demonstrate that estradiol regulates the amount of ERalpha in the membrane, suggesting estradiol can regulate its own membrane signaling. |
