| First Author | Kfoury N | Year | 2009 |
| Journal | Mol Cell Neurosci | Volume | 40 |
| Issue | 3 | Pages | 313-27 |
| PubMed ID | 19103292 | Mgi Jnum | J:146920 |
| Mgi Id | MGI:3838920 | Doi | 10.1016/j.mcn.2008.11.004 |
| Citation | Kfoury N, et al. (2009) Identification of neuronal target genes for CCAAT/enhancer binding proteins. Mol Cell Neurosci 40(3):313-27 |
| abstractText | CCAAT/Enhancer Binding Proteins (C/EBPs) play pivotal roles in the development and plasticity of the nervous system. Identification of the physiological targets of C/EBPs (C/EBP target genes) should therefore provide insight into the underlying biology of these processes. We used unbiased genome-wide mapping to identify 115 C/EBPbeta target genes in PC12 cells that include transcription factors, neurotransmitter receptors, ion channels, protein kinases and synaptic vesicle proteins. C/EBPbeta binding sites were located primarily within introns, suggesting novel regulatory functions, and were associated with binding sites for other developmentally important transcription factors. Experiments using dominant negatives showed C/EBPbeta to repress transcription of a subset of target genes. Target genes in rat brain were subsequently found to preferentially bind C/EBPalpha, beta and delta. Analysis of the hippocampal transcriptome of C/EBPbeta knockout mice revealed dysregulation of a high percentage of transcripts identified as C/EBP target genes. These results support the hypothesis that C/EBPs play non-redundant roles in the brain. |
