| First Author | Satake S | Year | 2012 |
| Journal | J Immunol | Volume | 189 |
| Issue | 9 | Pages | 4546-55 |
| PubMed ID | 23024276 | Mgi Jnum | J:190605 |
| Mgi Id | MGI:5449292 | Doi | 10.4049/jimmunol.1103007 |
| Citation | Satake S, et al. (2012) C/EBPbeta is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis. J Immunol 189(9):4546-55 |
| abstractText | Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein beta (C/EBPbeta) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPbeta is required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations (#1-5) according to their levels of c-Kit and Ly-6G expression. After the induction of candidemia, rapid mobilization of mature granulocytes and an increase in early granulocyte precursors accompanied by cell cycle acceleration was followed by a gradual increase in granulocytes originating from the immature populations. Upon infection, C/EBPbeta was upregulated at the protein level in all the granulopoietic subpopulations. The rapid increase in immature subpopulations #1 and #2 observed in C/EBPbeta knockout mice at 1 d postinfection was attenuated. Candidemia-induced cell cycle acceleration and proliferation of hematopoietic stem/progenitors were also impaired. Taken together, these data suggest that C/EBPbeta is involved in the efficient amplification of early granulocyte precursors during candidemia-induced emergency granulopoiesis. |
