| First Author | Tsai ELS | Year | 2019 |
| Journal | Stem Cells | Volume | 37 |
| Issue | 4 | Pages | 529-541 |
| PubMed ID | 30715780 | Mgi Jnum | J:291310 |
| Mgi Id | MGI:6446459 | Doi | 10.1002/stem.2985 |
| Citation | Tsai ELS, et al. (2019) Modeling of Photoreceptor Donor-Host Interaction Following Transplantation Reveals a Role for Crx, Muller Glia, and Rho/ROCK Signaling in Neurite Outgrowth. Stem Cells 37(4):529-541 |
| abstractText | The goal of photoreceptor transplantation is to establish functional synaptic connectivity between donor cells and second-order neurons in the host retina. There is, however, limited evidence of donor-host photoreceptor connectivity post-transplant. In this report, we investigated the effect of the host retinal environment on donor photoreceptor neurite outgrowth in vivo and identified a neurite outgrowth-promoting effect of host Crx((-/-)) retinas following transplantation of purified photoreceptors expressing green fluorescent protein (GFP). To investigate the noncell autonomous factors that influence donor cell neurite outgrowth in vitro, we established a donor-host coculture system using postnatal retinal aggregates. Retinal cell aggregation is sensitive to several factors, including plate coating substrate, cell density, and the presence of Muller glia. Donor photoreceptors exhibit motility in aggregate cultures and can engraft into established aggregate structures. The neurite outgrowth-promoting phenotype observed in Crx((-/-)) recipients in vivo is recapitulated in donor-host aggregate cocultures, demonstrating the utility of this surrogate in vitro approach. The removal of Muller glia from host aggregates reduced donor cell neurite outgrowth, identifying a role for this cell type in donor-host signaling. Although disruption of chondroitin sulfate proteoglycans in aggregates had no effect on the neurite outgrowth of donor photoreceptors, disruption of Rho/ROCK signaling enhanced outgrowth. Collectively, these data show a novel role of Crx, Muller glia, and Rho/ROCK signaling in controlling neurite outgrowth and provide an accessible in vitro model that can be used to screen for factors that regulate donor-host connectivity. Stem Cells 2019;37:529-541. |
