| First Author | Han JYS | Year | 2020 |
| Journal | FASEB J | Volume | 34 |
| Issue | 4 | Pages | 5401-5419 |
| PubMed ID | 32112484 | Mgi Jnum | J:287641 |
| Mgi Id | MGI:6416773 | Doi | 10.1096/fj.201902961R |
| Citation | Han JYS, et al. (2020) Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion. FASEB J 34(4):5401-5419 |
| abstractText | The neural retina metabolizes glucose through aerobic glycolysis generating large amounts of lactate. Lactate flux into and out of cells is regulated by proton-coupled monocarboxylate transporters (MCTs), which are encoded by members of the Slc16a family. MCT1, MCT3, and MCT4 are expressed in the retina and require association with the accessory protein basigin, encoded by Bsg, for maturation and trafficking to the plasma membrane. Bsg(-/-) mice have severely reduced electroretinograms (ERGs) and progressive photoreceptor degeneration, which is presumed to be driven by metabolic dysfunction resulting from loss of MCTs. To understand the basis of the Bsg(-/-) phenotype, we generated mice with conditional deletion of Bsg in rods (RodDeltaBsg), cones (ConeBsg), or retinal pigment epithelial cells (RPEDeltaBsg). RodDeltaBsg mice showed a progressive loss of photoreceptors, while ConeDeltaBsg mice did not display a degenerative phenotype. The RPEDeltaBsg mice developed a distinct phenotype characterized by severely reduced ERG responses as early as 4 weeks of age. The loss of lactate transporters from the RPE most closely resembled the phenotype of the Bsg(-/-) mouse, suggesting that the regulation of lactate levels in the RPE and the subretinal space is essential for the viability and function of photoreceptors. |
