| First Author | Kanan Y | Year | 2008 |
| Journal | Exp Eye Res | Volume | 86 |
| Issue | 2 | Pages | 344-54 |
| PubMed ID | 18163989 | Mgi Jnum | J:132505 |
| Mgi Id | MGI:3776181 | Doi | 10.1016/j.exer.2007.11.006 |
| Citation | Kanan Y, et al. (2008) Retinoid processing in cone and Muller cell lines. Exp Eye Res 86(2):344-54 |
| abstractText | To determine whether cones and Muller cells in the rod dominated retina cooperate to regenerate the 11-cis retinal chromophore via the retinoid cycle, two cell lines from the rod dominated retinas of Murine were used for this study: 661W, a mouse cell line derived from cones, and rMC-1, a rat Muller cell line. Retinoid cycle enzymes were analyzed by RT-PCR, and their catalytic activity was detected by incubation with retinoids and analyzed by HPLC. We found that 661W cells are capable of reducing all-trans retinal to all-trans retinol due to the presence of multiple dehydrogenases and to generate minor amounts of retinyl-ester. The rMC-1 cells take up all-trans retinol and oxidize it to all-trans retinal or esterify it to retinyl-ester, but are incapable of isomerizing all-trans retinoids to 11-cis retinoids. This could be a reflection of lack of necessary activities in Muller cells in vivo, which suggests that Muller cells do not contribute to retinoid cycling by regenerating 11-cis retinoids. Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids. |
