| First Author | Frampton G | Year | 2020 |
| Journal | Toxicol Appl Pharmacol | Volume | 409 |
| Pages | 115323 | PubMed ID | 33176120 |
| Mgi Jnum | J:301129 | Mgi Id | MGI:6502890 |
| Doi | 10.1016/j.taap.2020.115323 | Citation | Frampton G, et al. (2020) Inhibition of thrombospondin-1 reduces glutathione activity and worsens acute liver injury during acetaminophen hepatotoxicity in mice. Toxicol Appl Pharmacol 409:115323 |
| abstractText | Acetaminophen (N-Acetyl-p-Aminophenol or APAP)-induced hepatotoxicity is the most common cause of acute liver failure in the United States and Western Europe. Previous studies have shown that TGFbeta1 is elevated during APAP-induced hepatotoxicity and promotes liver injury by reducing liver regeneration while inducing hepatocyte senescence. At this time, little is known about the role of proteins that activate latent TGFbeta1 and their effects during APAP-induced hepatotoxicity. Thrombospondin-1 (TSP1) is a homotrimeric protein that can not only activate latent TGFbeta1 but can also interact with other proteins including Nrf2 to induce antioxidant signaling. The aim of the current study was to assess the role of thrombospondin-1 (TSP1) in both TGFbeta1 activation and its contribution to APAP-induced liver injury. C57Bl/6 mice or TSP1 null mice (TSP1(-/-)) were administered 300 mg/kg or 600 mg/kg of APAP. TGFbeta1 signaling, TSP1 expression, measures of hepatic injury, Nrf2 expression, measures of oxidative/nitrosative stress and GSH metabolism were assessed. The expression of TGFbeta1, TSP1 and phosphorylation of SMAD proteins increased in APAP-treated mice compared to controls. TSP1(-/-) mice had reduced TGFbeta1 expression and phosphorylation of SMAD proteins but increased liver injury. Hepatocyte cell death was increased in TSP1(-/-) mice and this was associated with decreased Nrf2 activity, decreased GSH levels and increased oxidative stress in comparison to wild-type C57Bl/6 mice. Together, these data demonstrate that elimination of TSP1 protein in APAP-treated mice reduces TGFbeta1 signaling but leads to increased liver injury by reducing Nrf2 expression and GSH activity, ultimately resulting in increased cell death. |
