| First Author | Hogenbirk MA | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 7 | Pages | e69815 |
| PubMed ID | 23922811 | Mgi Jnum | J:204695 |
| Mgi Id | MGI:5538445 | Doi | 10.1371/journal.pone.0069815 |
| Citation | Hogenbirk MA, et al. (2013) Differential programming of B cells in AID deficient mice. PLoS One 8(7):e69815 |
| abstractText | The Aicda locus encodes the activation induced cytidine deaminase (AID) and is highly expressed in germinal center (GC) B cells to initiate somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin (Ig) genes. Besides these Ig specific activities in B cells, AID has been implicated in active DNA demethylation in non-B cell systems. We here determined a potential role of AID as an epigenetic eraser and transcriptional regulator in B cells. RNA-Seq on different B cell subsets revealed that Aicda(-/-) B cells are developmentally affected. However as shown by RNA-Seq, MethylCap-Seq, and SNP analysis these transcriptome alterations may not relate to AID, but alternatively to a CBA mouse strain derived region around the targeted Aicda locus. These unexpected confounding parameters provide alternative, AID-independent interpretations on genotype-phenotype correlations previously reported in numerous studies on AID using the Aicda(-/-) mouse strain. |
