| First Author | Shirane M | Year | 2001 |
| Journal | Development | Volume | 128 |
| Issue | 24 | Pages | 5173-80 |
| PubMed ID | 11748152 | Mgi Jnum | J:73254 |
| Mgi Id | MGI:2154809 | Doi | 10.1242/dev.128.24.5173 |
| Citation | Shirane M, et al. (2001) Deficiency of phospholipase C-gamma1 impairs renal development and hematopoiesis. Development 128(24):5173-80 |
| abstractText | Phospholipase C-gamma1 (PLC-gamma1) is involved in a variety of intracellular signaling via many growth factor receptors and T-cell receptor. To explore the role of PLC-gamma1 in vivo, we generated the PLC-gamma1-deficient (plc-gamma1(-/-)) mice, which died of growth retardation at embryonic day 8.5-9.5 in utero. Therefore, we examined plc-gamma1(-/-) chimeric mice generated with plc-gamma1(-/-) embryonic stem (ES) cells for further study. Pathologically, plc-gamma1(-/-) chimeras showed multicystic kidney due to severe renal dysplasia and renal tube dilation. Flow cytometric analysis and glucose phosphate isomerase assay revealed very few hematopoietic cells derived from the plc-gamma1(-/-) ES cells in the mutant chimeras. However, differentiation of plc-gamma1(-/-) ES cells into erythrocytes and monocytes/macrophages in vitro was observed to a lesser extent compared with control wild-type ES cells. These data suggest that PLC-gamma1 plays an essential role in the renal development and hematopoiesis in vivo. |
