| First Author | Postupna N | Year | 2017 |
| Journal | Am J Pathol | Volume | 187 |
| Issue | 4 | Pages | 884-895 |
| PubMed ID | 28212814 | Mgi Jnum | J:241590 |
| Mgi Id | MGI:5903159 | Doi | 10.1016/j.ajpath.2016.12.010 |
| Citation | Postupna N, et al. (2017) Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE epsilon4. Am J Pathol 187(4):884-895 |
| abstractText | Cognitive impairment in older individuals is a complex trait that in population-based studies most commonly derives from an individually varying mixture of Alzheimer disease, Lewy body disease, and vascular brain injury. We investigated the molecular composition of synaptic particles from three sources: consecutive rapid autopsy brains from the Adult Changes in Thought Study, a population-based cohort; four aged nonhuman primate brains optimally processed for molecular investigation; and targeted replacement transgenic mice homozygous for APOE epsilon4. Our major goal was to characterize the molecular composition of human synaptic particles in regions of striatum and prefrontal cortex. We performed flow cytometry to measure six markers of synaptic subtypes, as well as amyloid beta 42 and paired helical filament tau. Our results showed selective degeneration of dopaminergic terminals throughout the striatum in individuals with Lewy body disease, and serotonergic degeneration in human ventromedial caudate nucleus from individuals with an APOE epsilon4 allele. Similar results were seen in mouse caudate nucleus homozygous for APOE epsilon4 via targeted replacement. Together, extension of these clinical, pathologic, and genetic associations from tissue to the synaptic compartment of cerebral cortex and striatum strongly supports our approach for accurately observing the molecular composition of human synapses by flow cytometry. |
