Primary Identifier | MGI:2157061 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence | Gene | Otx2 |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An Emx2 cDNA was inserted into the Otx2 gene as follows: the Emx2 cDNA was inserted at the start codon of the Otx2 gene followed by a loxP-flanked neomycin selection cassette. Resulting chimeric mice were bred to females expressing Cre in resulting zygotes, and the F1 generation was screened for the removal of the neomycin selection cassette. |