| First Author | Wang F | Year | 2023 |
| Journal | Nutrients | Volume | 15 |
| Issue | 22 | PubMed ID | 38004234 |
| Mgi Jnum | J:355567 | Mgi Id | MGI:7719093 |
| Doi | 10.3390/nu15224840 | Citation | Wang F, et al. (2023) Apolipoprotein A-IV-Deficient Mice in 129/SvJ Background Are Susceptible to Obesity and Glucose Intolerance. Nutrients 15(22) |
| abstractText | Apolipoprotein A-IV (apoA-IV), synthesized by enterocytes, is potentially involved in regulating lipid absorption and metabolism, food intake, and glucose metabolism. In this study, we backcrossed apoA-IV knockout (apoA-IV(-/-)) mice onto the 129/SvJ background for eight generations. Compared to the wild-type (WT) mice, the 129/SvJ apoA-IV(-/-) mice gained more weight and exhibited delayed glucose clearance even on the chow diet. During a 16-week high-fat diet (20% by weight of fat) study, apoA-IV(-/-) mice were more obese than the WT mice, which was associated with their increased food intake as well as reduced energy expenditure and physical activity. In addition, apoA-IV(-/-) mice developed significant insulin resistance (indicated by HOMA-IR) with severe glucose intolerance even though their insulin levels were drastically higher than the WT mice. In conclusion, we have established a model of apoA-IV(-/-) mice onto the 129/SvJ background. Unlike in the C57BL/6J strain, apoA-IV(-/-) 129/SvJ mice become significantly more obese and insulin-resistant than WT mice. Our current investigations of apoA-IV in the 129/SvJ strain and our previous studies in the C57BL/6J strain underline the impact of genetic background on apoA-IV metabolic effects. |
