| First Author | Petit M | Year | 2024 |
| Journal | Cell Rep | Volume | 43 |
| Issue | 10 | Pages | 114847 |
| PubMed ID | 39395172 | Mgi Jnum | J:358154 |
| Mgi Id | MGI:7779372 | Doi | 10.1016/j.celrep.2024.114847 |
| Citation | Petit M, et al. (2024) Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ. Cell Rep 43(10):114847 |
| abstractText | Tissue-resident mononuclear phagocytes (MPs) are an abundant cell population whose localization in situ reflects their identity. To enable assessment of their heterogeneity, we developed the red/green/blue (RGB)-Mac mouse based upon combinations of Cx3cr1 and Csf1r reporter transgenes, providing a complete visualization of their spatial organization in situ. 3D-multi-photon imaging for spatial mapping and spectral cytometry employing the three markers in combination distinguished tissue-associated monocytes, tissue-specific macrophages, and three subsets of connective-tissue-associated MPs, including CCR2(+) monocyte-derived cell, CX3CR1(+), and FOLR2(+) interstitial subsets, associated with distinct sub-anatomic territories. These populations were selectively reduced by blockade of CSF1, CSF2, CCR2, and CX3CR1 and efficiently reconstitute their spatial distribution after transient myelo-ablation, suggesting an autonomous regulatory environment. Our findings emphasize the organization of the MP compartment at the sub-anatomic level under steady-state conditions, thereby providing a holistic understanding of their relative heterogeneity across different tissues. |
