| First Author | Sambhara S | Year | 1998 |
| Journal | Cell Immunol | Volume | 187 |
| Issue | 1 | Pages | 13-8 |
| PubMed ID | 9681998 | Mgi Jnum | J:114193 |
| Mgi Id | MGI:3688480 | Doi | 10.1006/cimm.1998.1314 |
| Citation | Sambhara S, et al. (1998) Enhanced antibody and cytokine responses to influenza viral antigens in perforin-deficient mice. Cell Immunol 187(1):13-8 |
| abstractText | Cytotoxic T lymphocytes (CTL) lyse virus-infected target cells by secreting the pore-forming effector molecule, perforin. Perforin-mediated cell death appears to be a major mechanism in viral clearance but its role in regulating immune responses in vivo is unclear. In this report, we show that following immunization with influenza viral antigens, perforin-deficient mice generated about 100-fold greater serum antibody responses than wild-type mice. Further, immune spleen cells from perforin knock-out mice secreted over 10-fold more IFN-gamma following in vitro restimulation than immune spleen cells from control mice. Finally, there were over 10-fold more IFN-gamma-secreting cells in cultures from perforin-deficient mice than those from control mice, suggesting that the enhanced cytokine release by T cells from perforin-deficient mice is due to an increase in the effector cell pool. Collectively, these results suggest that perforin-mediated effector function is required in the down-regulation of the immune response by way of limiting antigen-presenting cell function. |
