| First Author | Ho NI | Year | 2017 |
| Journal | J Immunol | Volume | 198 |
| Issue | 11 | Pages | 4235-4243 |
| PubMed ID | 28432146 | Mgi Jnum | J:247864 |
| Mgi Id | MGI:5927344 | Doi | 10.4049/jimmunol.1602169 |
| Citation | Ho NI, et al. (2017) C1q-Dependent Dendritic Cell Cross-Presentation of In Vivo-Formed Antigen-Antibody Complexes. J Immunol 198(11):4235-4243 |
| abstractText | Dendritic cells (DCs) are specialized in Ag engulfment via a wide variety of uptake receptors on their cell surface. In the present study we investigated Ag uptake and presentation of in vivo-formed Ag-Ab complexes by i.v. injecting mice with Ag-specific Abs followed by the cognate Ag. We show by this natural Ab-mediated Ag targeting system that uptake by splenic APC subsets is severely hampered in mice lacking complement factor C1q (C1qa-/-). Moreover, no detectable Ag cross-presentation by CD8alpha+ DCs from C1qa-/- mice was found. On the contrary, Ag uptake was not hampered by APCs in FcgammaRI/II/III/IV-deficient (FcgammaR quadruple-/-) mice, and the cross-presentation ability of CD8alpha+ DCs was not affected. In conclusion, we show that C1q rather than FcgammaRs controls the Ab-mediated Ag uptake and its presentation by spleen APC subsets to T cells. |
