| First Author | Compan V | Year | 2012 |
| Journal | Immunity | Volume | 37 |
| Issue | 3 | Pages | 487-500 |
| PubMed ID | 22981536 | Mgi Jnum | J:187661 |
| Mgi Id | MGI:5437762 | Doi | 10.1016/j.immuni.2012.06.013 |
| Citation | Compan V, et al. (2012) Cell Volume Regulation Modulates NLRP3 Inflammasome Activation. Immunity 37(3):487-500 |
| abstractText | Cell volume regulation is a primitive response to alterations in environmental osmolarity. The NLRP3 inflammasome is a multiprotein complex that senses pathogen- and danger-associated signals. Here, we report that, from fish to mammals, the basic mechanisms of cell swelling and regulatory volume decrease (RVD) are sensed via the NLRP3 inflammasome. We found that a decrease in extracellular osmolarity induced a K(+)-dependent conformational change of the preassembled NLRP3-inactive inflammasome during cell swelling, followed by activation of the NLRP3 inflammasome and caspase-1, which was controlled by transient receptor potential channels during RVD. Both mechanisms were necessary for interleukin-1beta processing. Increased extracellular osmolarity prevented caspase-1 activation by different known NLRP3 activators. Collectively, our data identify cell volume regulation as a basic conserved homeostatic mechanism associated with the formation of the NLRP3 inflammasome and reveal a mechanism for NLRP3 inflammasome activation. |
