| First Author | Bosma GC | Year | 2002 |
| Journal | J Exp Med | Volume | 196 |
| Issue | 11 | Pages | 1483-95 |
| PubMed ID | 12461083 | Mgi Jnum | J:118010 |
| Mgi Id | MGI:3698350 | Doi | 10.1084/jem.20001871 |
| Citation | Bosma GC, et al. (2002) DNA-dependent protein kinase activity is not required for immunoglobulin class switching. J Exp Med 196(11):1483-95 |
| abstractText | Class switch recombination (CSR), similar to V(D)J recombination, is thought to involve DNA double strand breaks and repair by the nonhomologous end-joining pathway. A key component of this pathway is DNA-dependent protein kinase (DNA-PK), consisting of a catalytic subunit (DNA-PKcs) and a DNA-binding heterodimer (Ku70/80). To test whether DNA-PKcs activity is essential for CSR, we examined whether IgM(+) B cells from scid mice with site-directed H and L chain transgenes were able to undergo CSR. Although B cells from these mice were shown to lack DNA-PKcs activity, they were able to switch from IgM to IgG or IgA with close to the same efficiency as B cells from control transgenic and nontransgenic scid/+ mice, heterozygous for the scid mutation. We conclude that CSR, unlike V(D)J recombination, can readily occur in the absence of DNA-PKcs activity. We suggest nonhomologous end joining may not be the (primary or only) mechanism used to repair DNA breaks during CSR. |
