| First Author | Peña JR | Year | 2004 |
| Journal | Cardiovasc Res | Volume | 61 |
| Issue | 4 | Pages | 756-63 |
| PubMed ID | 14985072 | Mgi Jnum | J:134598 |
| Mgi Id | MGI:3789396 | Doi | 10.1016/j.cardiores.2003.12.019 |
| Citation | Pena JR, et al. (2004) Troponin I phosphorylation plays an important role in the relaxant effect of beta-adrenergic stimulation in mouse hearts. Cardiovasc Res 61(4):756-63 |
| abstractText | OBJECTIVE: The present study was designed to address the question of the contribution of cardiac troponin I (cTnI) phosphorylation to the enhanced rate of relaxation during beta-adrenergic stimulation in hearts in situ. METHODS: In situ hemodynamic measurements were performed in mouse hearts that (1) express normal level of phospholamban (PLB) and either express cTnI (PLB/cTnI) or the slow skeletal isoform of TnI (PLB/ssTnI) that cannot be phosphorylated by protein kinase A (PKA) or (2) do not express PLB and either express cTnI (PLBKO/cTnI) or ssTnI (PLBKO/ssTnI). RESULTS: In the basal state, there was no difference in heart rate (HR), developed pressure (DP), left ventricular end-diastolic pressure (LVEDP) or rate of contraction (+dP/dt) between PLB/cTnI and PLB/ssTnI groups. However, hearts expressing ssTnI (PLB/ssTnI) showed a significantly decreased rate of relaxation (-dP/dt) when compared with hearts expressing cTnI (PLB/cTnI). In response to beta-adrenergic agonist, isoproterenol (ISO), HR increased similarly in both groups. At the two highest doses of ISO, the rate of relaxation (-dP/dt) was significantly smaller in PLB/ssTnI than in PLB/cTnI hearts. In the basal state, there was no difference in HR, DP, LVEDP,+dP/dt and -dP/dt between PLBKO/cTnI and PLBKO/ssTnI hearts. In response to ISO, HR increased similarly in both groups and was only slightly smaller in PLBKO/ssTnI group at the lowest dose of ISO. However, during ISO perfusion, when cTnI was phosphorylated, the rate of relaxation was significantly slower in PLBKO/ssTnI compared to PLBKO/cTnI hearts. CONCLUSION: Our data support the hypothesis that phosphorylation of cTnI significantly contributes to the enhanced rate of relaxation during beta-adrenergic stimulation. |
