Primary Identifier | MGI:2158361 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Traf4 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A genomic fragment containing exons 2-7 was replaced with a neomycin selection cassette inserted in reverse transcriptional orientation to the gene, leaving part of the RING finger (exon 1). Northern blot analysis on tissues derived from homozygous mice confirmed that to detectable transcript was produced from this allele. Western blot analysis on protein extracts from various tissues of homozygous mice demonstrated that no detectable protein was made from this allele. |