| First Author | Yukata K | Year | 2018 |
| Journal | Bone | Volume | 110 |
| Pages | 150-159 | PubMed ID | 29408411 |
| Mgi Jnum | J:262531 | Mgi Id | MGI:6161077 |
| Doi | 10.1016/j.bone.2018.02.001 | Citation | Yukata K, et al. (2018) Teriparatide (human PTH1-34) compensates for impaired fracture healing in COX-2 deficient mice. Bone 110:150-159 |
| abstractText | Genetic ablation of cyclooxygenase-2 (COX-2) in mice is known to impair fracture healing. To determine if teriparatide (human PTH1-34) can promote healing of Cox-2-deficient fractures, we performed detailed in vivo analyses using a murine stabilized tibia fracture model. Periosteal progenitor cell proliferation as well as bony callus formation was markedly reduced in Cox-2(-/-) mice at day 10 post-fracture. Remarkably, intermittent PTH1-34 administration increased proliferation of periosteal progenitor cells, restored callus formation on day 7, and enhanced bone formation on days 10, 14 and 21 in Cox-2-deficient mice. PTH1-34 also increased biomechanical torsional properties at days 10 or 14 in all genotypes, consistent with enhanced bony callus formation by radiologic examinations. To determine the effects of intermittent PTH1-34 for callus remodeling, TRAP staining was performed. Intermittent PTH1-34 treatment increased the number of TRAP positive cells per total callus area on day 21 in Cox-2(-/-) fractures. Taken together, the present findings indicate that intermittent PTH1-34 treatment could compensate for COX-2 deficiency and improve impaired fracture healing in Cox-2-deficient mice. |
