| First Author | Mai HN | Year | 2020 |
| Journal | Brain Res Bull | Volume | 164 |
| Pages | 107-120 | PubMed ID | 32822804 |
| Mgi Jnum | J:298231 | Mgi Id | MGI:6477465 |
| Doi | 10.1016/j.brainresbull.2020.08.011 | Citation | Mai HN, et al. (2020) Glutathione peroxidase-1 knockout potentiates behavioral sensitization induced by cocaine in mice via sigma-1 receptor-mediated ERK signaling: A comparison with the case of glutathione peroxidase-1 overexpressing transgenic mice. Brain Res Bull 164:107-120 |
| abstractText | We demonstrated that the gene of glutathione peroxidase-1 (GPx-1), a major antioxidant enzyme, is a potential protectant against the neurotoxicity and conditioned place preference induced by cocaine. Because the sigma (sigma)-1 receptor is implicated in cocaine-induced drug dependence, we investigated whether the GPx-1 gene modulates the sigma-1 receptor in the behavioral sensitization induced by cocaine. Cocaine-induced behavioral sensitization was more pronounced in GPx-1 knockout (KO) than wild-type (WT) mice and was less pronounced in GPx-1 overexpressing transgenic (GPx-1 TG) than non-TG mice. Cocaine treatment significantly enhanced the oxidative burden and reduced the GSH levels in the striatum of WT, GPx-1 KO, and non-TG mice but not in that of GPx-1 TG mice. In addition, cocaine significantly increased the nuclear translocation, its DNA binding activity of nuclear factor erythroid-2-related factor 2 (Nrf2) as well as the mRNA expression of gamma-glutamylcysteine (GCL). The genetic depletion of GPx-1 inhibited the Nrf2-related glutathione system, whereas the genetic overexpression of GPx-1 activated this system against behavioral sensitization. BD1047, a sigma-1 receptor antagonist, and U0126, an ERK inhibitor significantly induced the Nrf2-related antioxidant potential against behavioral sensitization. Unlike BD1047, U0126 did not affect the cocaine-induced sigma-1 receptor immunoreactivity, suggesting that the sigma-1 receptor is an upstream molecule for ERK signaling. Importantly, BD1047 and U0126 failed to affect the sigma-1 receptor immunoreactivity and ERK phosphorylation induced by cocaine in GPx-1 TG mice. Our results suggest that GPx-1 is a critical mediator for the attenuation of cocaine-induced behavioral sensitization via modulating sigma-1 receptor-mediated ERK activation by the induction of the Nrf2-related system. |
