| First Author | Kolpakova-Hart E | Year | 2008 |
| Journal | Matrix Biol | Volume | 27 |
| Issue | 6 | Pages | 505-12 |
| PubMed ID | 18579360 | Mgi Jnum | J:140779 |
| Mgi Id | MGI:3814618 | Doi | 10.1016/j.matbio.2008.05.002 |
| Citation | Kolpakova-Hart E, et al. (2008) Col2-Cre recombinase is co-expressed with endogenous type II collagen in embryonic renal epithelium and drives development of polycystic kidney disease following inactivation of ciliary genes. Matrix Biol 27(6):505-12 |
| abstractText | Here we report on the severe defects in renal epithelium induced by the transgenic Col2-Cre line used previously for skeletal tissue-specific gene targeting. We demonstrate that conditional ablation of the Kif3a or Pkd1 genes encoding primary cilium/intraflagellar transport-associated proteins using type II collagen-specific Cre transgenic strain results in a severe form of polycystic kidney disease in mice. We detect Col2-Cre recombinase expression in kidney epithelium, which reflects expression of the endogenous Col1alpha(II) gene in the embryonic renal tubules. We determine the exon 2-containing splice variant of the Col1alpha(II) gene as a major transcript expressed in kidney. Furthermore, the confocal immunocytochemical analysis demonstrates deposition of the type II collagen within the mesenchymal-epithelial renal tissue interfaces and its co-localization with the basement membrane marker collagen IV during embryonic kidney morphogenesis. |
