| Primary Identifier | MGI:2176279 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Reporter | Gene | Tg(CAG-cat,-lacZ)34Pva |
| Strain of Origin | (C57BL/6 x DBA/2)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Three of 12 transgenic mice derived from oocyte injection were found to produce F1 progeny expressing high levels of chloramphenicol acetyltransferase (CAT) in the heart. Line 34, estimated to carry 4 copies of the transgene, was selected for propagation and experimental use because it was the most fertile. PCR analysis of tissues of mice generated from fertilized oocytes in which Cre recombinase was transiently expressed demonstrated efficient excision of the chloramphenicol acetyltransferase gene. Beta-galactosidase activity is very high in muscle and heart of these mice, but not above background elsewhere. |
| molecularNote | This Cre recombinase reporter transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from the bacterial beta-galactosidase (lacZ) coding sequence, with a rabbit globin polyadenylation signal, by a loxP-flanked chloramphenicol acetyltransferase (cat) gene with the SV40 small t intron and polyadenylation signal. ELISA demonstrated high-level expression of CAT protein in heart and muscle and lower levels in other tissues of transgenic mice. |
