| First Author | Bennett MK | Year | 2010 |
| Journal | Mol Cell Neurosci | Volume | 43 |
| Issue | 4 | Pages | 353-62 |
| PubMed ID | 20080187 | Mgi Jnum | J:158375 |
| Mgi Id | MGI:4438667 | Doi | 10.1016/j.mcn.2010.01.002 |
| Citation | Bennett MK, et al. (2010) Odor-evoked gene regulation and visualization in olfactory receptor neurons. Mol Cell Neurosci 43(4):353-62 |
| abstractText | Odorant-evoked activity contributes to olfactory epithelium organization and axon targeting. We examined the consequences on gene expression of a genetic disruption of the channel responsible for olfactory transduction. Genes encoding calcium-binding EF-hand motifs, were among the most highly regulated transcripts consistent with the central role of Ca(2+) influx in neuronal depolarization. Several genes encoding integral membrane proteins are also highly regulated. One gene, Lrrc3b, was regulated more than 10-fold by odorant activity. Changes in expression occur within thirty minutes and are maintained for several hours. In genetic disruptions of Lrrc3b, a Lrrc3b-promoter-driven reporter adopts the activity-regulated expression of the endogenous gene. Individual olfactory glomeruli have a wide spectrum of activity levels that can be modulated by altering odor exposure. The Lrrc3b reporter mouse permits direct assessment of activity in identified glomeruli. In stable odorant environments, activity-regulated proteins provide a characteristic signature that is correlated with the olfactory receptor they express. |
