| Primary Identifier | MGI:2159360 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Fadd |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Authors in J:46527 state that injection of mutant ES cells into Gt(ROSA)26Sor E3.5 blastocysts (J:79478) resulting in high percentage chimeric embryos with a high contribution of mutant cells to the heart reproduce the phenotype of homozygous mutant embryos derived from heterozygous breedings. In contrast, chimeric embryos with a low contribution from the mutant ES cells display a normal phenotype at E11.5. |
| molecularNote | A neomycin selection cassette replaced a genomic fragment containing the entire coding region of the gene. Western blot analysis on lysates derived from homozygous mice confirmed that no detectable protein was expressed from this allele. |
