| First Author | Stumpf M | Year | 2010 |
| Journal | Proc Natl Acad Sci U S A | Volume | 107 |
| Issue | 50 | Pages | 21541-6 |
| PubMed ID | 21098667 | Mgi Jnum | J:167145 |
| Mgi Id | MGI:4867338 | Doi | 10.1073/pnas.1005794107 |
| Citation | Stumpf M, et al. (2010) Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1. Proc Natl Acad Sci U S A 107(50):21541-6 |
| abstractText | The Mediator complex forms the bridge between transcriptional activators and the RNA polymerase II. Med1 (also known as PBP or TRAP220) is a key component of Mediator that interacts with nuclear hormone receptors and GATA transcription factors. Here, we show dynamic recruitment of GATA-1, TFIIB, Mediator, and RNA polymerase II to the beta-globin locus in induced mouse erythroid leukemia cells and in an erythropoietin-inducible hematopoietic progenitor cell line. Using Med1 conditional knockout mice, we demonstrate a specific block in erythroid development but not in myeloid or lymphoid development, highlighted by the complete absence of beta-globin gene expression. Thus, Mediator subunit Med1 plays a pivotal role in erythroid development and in beta-globin gene activation. |
