| First Author | Ku CJ | Year | 2012 |
| Journal | Blood | Volume | 119 |
| Issue | 10 | Pages | 2242-51 |
| PubMed ID | 22267605 | Mgi Jnum | J:182538 |
| Mgi Id | MGI:5315814 | Doi | 10.1182/blood-2011-07-366070 |
| Citation | Ku CJ, et al. (2012) GATA-3 regulates hematopoietic stem cell maintenance and cell-cycle entry. Blood 119(10):2242-51 |
| abstractText | Maintaining hematopoietic stem cell (HSC) quiescence is a critical property for the life-long generation of blood cells. Approximately 75% of cells in a highly enriched long-term repopulating HSC (LT-HSC) pool (Lin(-)Sca1(+)c-Kit(hi)CD150(+)CD48(-)) are quiescent, with only a small percentage of the LT-HSCs in cycle. Transcription factor GATA-3 is known to be vital for the development of T cells at multiple stages in the thymus and for Th2 differentiation in the peripheral organs. Although it is well documented that GATA-3 is expressed in HSCs, a role for GATA-3 in any prethymic progenitor cell has not been established. In the present study, we show that Gata3-null mutant mice generate fewer LT-HSCs and that fewer Gata3-null LT-HSCs are in cycle. Furthermore, Gata3 mutant hematopoietic progenitor cells fail to be recruited into an increased cycling state after 5-fluorouracil-induced myelosuppression. Therefore, GATA-3 is required for the maintenance of a normal number of LT-HSCs and for their entry into the cell cycle. |
