| First Author | Karpowicz P | Year | 2009 |
| Journal | J Neurosci | Volume | 29 |
| Issue | 12 | Pages | 3885-96 |
| PubMed ID | 19321785 | Mgi Jnum | J:147274 |
| Mgi Id | MGI:3840014 | Doi | 10.1523/JNEUROSCI.0037-09.2009 |
| Citation | Karpowicz P, et al. (2009) E-Cadherin regulates neural stem cell self-renewal. J Neurosci 29(12):3885-96 |
| abstractText | E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells. |
