| First Author | Bissen D | Year | 2021 |
| Journal | Cell Rep | Volume | 34 |
| Issue | 13 | Pages | 108923 |
| PubMed ID | 33789115 | Mgi Jnum | J:304276 |
| Mgi Id | MGI:6694817 | Doi | 10.1016/j.celrep.2021.108923 |
| Citation | Bissen D, et al. (2021) EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity. Cell Rep 34(13):108923 |
| abstractText | Despite decades of work, much remains elusive about molecular events at the interplay between physiological and structural changes underlying neuronal plasticity. Here, we combined repetitive live imaging and expansion microscopy in organotypic brain slice cultures to quantitatively characterize the dynamic changes of the intracellular versus surface pools of GluA2-containing alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) across the different dendritic spine types and the shaft during hippocampal homeostatic plasticity. Mechanistically, we identify ephrinB2 and glutamate receptor interacting protein (GRIP) 1 as mediating AMPAR relocation to the mushroom spine surface following lesion-induced denervation. Moreover, stimulation with the ephrinB2 specific receptor EphB4 not only prevents the lesion-induced disappearance of mushroom spines but is also sufficient to shift AMPARs to the surface and rescue spine recovery in a GRIP1 dominant-negative background. Thus, our results unravel a crucial role for ephrinB2 during homeostatic plasticity and identify a potential pharmacological target to improve dendritic spine plasticity upon injury. |
