| First Author | Tewari BP | Year | 2024 |
| Journal | Nat Neurosci | Volume | 27 |
| Issue | 8 | Pages | 1475-1488 |
| PubMed ID | 39020018 | Mgi Jnum | J:353072 |
| Mgi Id | MGI:7707514 | Doi | 10.1038/s41593-024-01714-3 |
| Citation | Tewari BP, et al. (2024) Astrocytes require perineuronal nets to maintain synaptic homeostasis in mice. Nat Neurosci 27(8):1475-1488 |
| abstractText | Perineuronal nets (PNNs) are densely packed extracellular matrices that cover the cell body of fast-spiking inhibitory neurons. PNNs stabilize synapses inhibiting synaptic plasticity. Here we show that synaptic terminals of fast-spiking interneurons localize to holes in the PNNs in the adult mouse somatosensory cortex. Approximately 95% of holes in the PNNs contain synapses and astrocytic processes expressing Kir4.1, glutamate and GABA transporters. Hence, holes in the PNNs contain tripartite synapses. In the adult mouse brain, PNN degradation causes an expanded astrocytic coverage of the neuronal somata without altering the axon terminals. The loss of PNNs impairs astrocytic transmitter and potassium uptake, resulting in the spillage of glutamate into the extrasynaptic space. Our data show that PNNs and astrocytes cooperate to contain synaptically released signals in physiological conditions. Their combined action is altered in mouse models of Alzheimer's disease and epilepsy where PNNs are disrupted. |
