| First Author | Jiang W | Year | 2013 |
| Journal | Int J Biol Sci | Volume | 10 |
| Issue | 1 | Pages | 33-42 |
| PubMed ID | 24391449 | Mgi Jnum | J:309368 |
| Mgi Id | MGI:6755760 | Doi | 10.7150/ijbs.7526 |
| Citation | Jiang W, et al. (2013) Identification of Tmem10 as a novel late-stage oligodendrocytes marker for detecting hypomyelination. Int J Biol Sci 10(1):33-42 |
| abstractText | Oligodendrocytes ensheath axons to form compact insulating multilamellar structures known as myelin. Tmem10 is a novel type I transmembrane glycoprotein that is highly expressed in oligodendrocytes and whose biological function remains largely unknown. Furthermore, the expression pattern of Tmem10 remains a matter of some controversy. Given the inconsistency of its expression pattern and the lack of validated specific antibodies, Tmem10 is not widely accepted as a marker for mature oligodendrocytes. As a means to solve these problems and to aid future studies of oligodendrocyte-associated diseases, we have generated a highly specific Tmem10 antibody. Using this Tmem10 antibody, we clarify that Tmem10 protein is firstly expressed at 2 weeks in the postnatal mouse brain with age-related increase, only in the central nervous system (CNS). We also reveal that Tmem10 is expressed specifically in late stage oligodendrocytes and later than MAG, a late-stage myelin marker. Finally, we show that Tmem10 co-expresses with MOG- and MBP-positive myelin fibers and is dramatically reduced in a hypomyelination mouse model. In conclusion, our study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis. |
