| First Author | Ippolito GC | Year | 2006 |
| Journal | J Exp Med | Volume | 203 |
| Issue | 6 | Pages | 1567-78 |
| PubMed ID | 16754718 | Mgi Jnum | J:124421 |
| Mgi Id | MGI:3721493 | Doi | 10.1084/jem.20052217 |
| Citation | Ippolito GC, et al. (2006) Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production. J Exp Med 203(6):1567-78 |
| abstractText | Tyrosine and glycine constitute 40% of complementarity determining region 3 of the immunoglobulin heavy chain (CDR-H3), the center of the classic antigen-binding site. To assess the role of D(H) RF1-encoded tyrosine and glycine in regulating CDR-H3 content and potentially influencing B cell function, we created mice limited to a single D(H) encoding asparagine, histidine, and arginines in RF1. Tyrosine and glycine content in CDR-H3 was halved. Bone marrow and spleen mature B cell and peritoneal cavity B-1 cell numbers were also halved, whereas marginal zone B cell numbers increased. Serum immunoglobulin G subclass levels and antibody titers to T-dependent and T-independent antigens all declined. Thus, violation of the conserved preference for tyrosine and glycine in D(H) RF1 alters CDR-H3 content and impairs B cell development and antibody production. |
