| First Author | Cadiz-Rivera B | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 5 | Pages | e92947 |
| PubMed ID | 24817273 | Mgi Jnum | J:216256 |
| Mgi Id | MGI:5608563 | Doi | 10.1371/journal.pone.0092947 |
| Citation | Cadiz-Rivera B, et al. (2014) The chromatin "landscape" of a murine adult beta-globin gene is unaffected by deletion of either the gene promoter or a downstream enhancer. PLoS One 9(5):e92947 |
| abstractText | In mammals, the complex tissue- and developmental-specific expression of genes within the beta-globin cluster is known to be subject to control by the gene promoters, by a locus control region (LCR) located upstream of the cluster, and by sequence elements located across the intergenic regions. Despite extensive investigation, however, the complement of sequences that is required for normal regulation of chromatin structure and gene expression within the cluster is not fully defined. To further elucidate regulation of the adult beta-globin genes, we investigate the effects of two deletions engineered within the endogenous murine beta-globin locus. First, we find that deletion of the beta2-globin gene promoter, while eliminating beta2-globin gene expression, results in no additional effects on chromatin structure or gene expression within the cluster. Notably, our observations are not consistent with competition among the beta-globin genes for LCR activity. Second, we characterize a novel enhancer located 3' of the beta2-globin gene, but find that deletion of this sequence has no effect whatsoever on gene expression or chromatin structure. This observation highlights the difficulty in assigning function to enhancer sequences identified by the chromatin "landscape" or even by functional assays. |
